Biologist (San Diego, CA)

Biologist

Biologist needs 5 years laboratory industry experienceBiologist requires Bachelor s degree in Biological Sciences needs to do respirator fit test before he starts and he will be working onsite familiar with in vitro assays such as ELISA based platforms and RNA isolation. experience with standard protein purification techniques including affinity ion exchange and size exclusion chromatography analysis of protein purity by SDS-page gel electrophoresis and determination of protein concentration by Bradford assay and OD 280nm. Familiar with AKTA PURE and related protein chromatography instruments Must exhibit the ability to solve problems be self-motivated and capable of working independently while contributing to a project team environment Highly organized and effective at multi-tasking with the ability to run several purification experiments simultaneously Attention to detail and ability to keep accurate records Conscientious with a strong work ethic Adherence to Good Research Practices Proficiency in column chromatography for proteins protein concentration and protein analytical techniques will be key to this position. Good written and oral communication skills and strong computer skills are essential Biologist duties Protein purification and analytics work including the hands-on execution of purification and or characterization of protein reagents for structural biology and biochemical assays. High-throughput purification of recombinant proteins for crystallization studies and biochemical assays using standard chromatographic methods and automated parallel processing systems Lysis of prokaryotic and eukaryotic cell pellets and clarification of lysates by centrifugation Protein purification by affinity ion exchange and size exclusion chromatography Analysis of protein purity using SDS-page gel electrophoresis Conduct experiments to identify and validate drug targets. Support assay design optimization validation and data analyses. Design and execute in vivo and cell biology experiments. Troubleshoot and analyze complex data in support of drug discovery projects. Presentation of results and conclusions both orally and written.